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  • Title: Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography.
    Author: Yasumitsu H.
    Journal: Methods Mol Biol; 2017; 1626():13-24. PubMed ID: 28608196.
    Abstract:
    To detect serine protease activity by zymography, casein and CBB stain have been used as a substrate and a detection procedure, respectively. Casein zymography has been using substrate concentration at 1 mg/mL and employing conventional CBB stain. Although ordinary casein zymography provides reproducible results, it has several disadvantages including time-consuming and relative low sensitivity. Improved casein zymography, RAMA casein zymography, is rapid and highly sensitive. RAMA casein zymography completes the detection process within 1 h after incubation and increases the sensitivity at least by tenfold. In addition to serine protease, the method also detects metalloprotease 7 (MMP7, Matrilysin) with high sensitivity.
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