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  • Title: Regular enzyme recovery enhances cellulase production by Trichoderma reesei in fed-batch culture.
    Author: Wang B, Jiang L, Bai H, Yong Q, Yu S.
    Journal: Biotechnol Lett; 2017 Oct; 39(10):1493-1498. PubMed ID: 28612265.
    Abstract:
    OBJECTIVE: To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals. RESULTS: Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3.5-4.5 g l-1. CONCLUSION: Enzyme recovery at regular intervals during fed-batch cellulase cultivation could protect the enzyme in the culture broth and enhance the enzyme production when the fungal cell concentration is maintained in a reasonable range.
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