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Title: [Correlation of matrix metalloproteinase-9 polymorphisms with chronic periodontitis in Uygur adults].
Author: Ma T, Li DD, Huang P, Zhao J.
Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2017 Jun 09; 52(6):360-366. PubMed ID: 28613058.
Abstract:
Objective: To investigate the association between matrix metalloproteinase-9 (MMP-9) polymorphisms and chronic periodontitis in Uygur adults. Methods: A total of 196 patients with chronic periodontitis and 97 healthy controls were selected from 2 500 Uygur people. Buccal swab samples were taken, the genomic DNA was extracted and the genotype distribution and allele frequency of MMP-9 were determined by PCR-restriction fragment length polymorphism (PCR-RFLP). The distribution of genotypes, allele frequencies and risk factors were analyzed by chi-square test and multiple logistic regression. Results: Significant difference was found between healthy controls and the mild periodontitis and moderate to severe periodontitis in the MMP-9 1562C/T CC genotype expression (χ(2)=9.901, P=0.002; χ(2)=13.397, P< 0.001), and detectable rate of MMP-9 1562C/T CC genotype in the three groups was 31.3%(30/96), 53.5% (53/99), 27.8%(27/97), respectively. The detectable rate of CT genotype expression in the three groups were 65.6% (63/96), 45.5% (45/99), 69.1% (67/97) respectively and there was significant difference between healthy controls and mild periodontitis and between the mild periodontitis and moderate to severe periodontitis (χ(2)=8.025, P=0.005; χ(2)=11.159, P<0.001). There was also significant difference in allele frequency between healthy controls and mild periodontitis and between mild periodontitis and moderate to severe periodontitis (χ(2)=6.270, P=0.012; χ(2)=8.184, P=0.004). Logistic analysis showed that age under 35 years old was the protective factor of chronic periodontitis (OR=0.061, 95% CI=0.035-0.108, P<0.001) while the male and CT genotype were the risk factors of chronic periodontitis (OR=2.392, 95%CI=1.496-3.819, P<0.001; OR=1.280, 95%CI=0.794-2.067, P=0.031). Conclusions: The susceptibility to chronic periodontitis in Uygur adults in Moyu county of Xinjiang is related to the age and gender and polymorphism of MMP-9. The age over 35 years old, male and CT genotype may be the risk factors of chronic periodontitis in Uygur adults. 目的： 探讨基质金属蛋白酶9 (matrix metalloproteinase-9, MMP-9) 1562C/T位点单核苷酸多态性（single nucleotide polymorphism，SNP）与新疆墨玉县维吾尔族成人慢性牙周炎易感性的关系。 方法： 2013年3至4月抽取墨玉县20个自然村，对2 500名调查者（均为维吾尔族，年龄18~65岁）进行口腔检查，将纳入对象分为健康对照组、牙周炎轻度及中重度组。抽取196例慢性牙周炎（其中包括牙周炎轻度组99例，中重度组97例）和96名健康对照者（健康对照组）的口腔颊黏膜拭子样本。提取全基因组DNA，采用PCR-限制性片段长度多态性技术检测MMP-9 1562C/T基因型分布，应用Kruskal-Wallis H检验分析组间一般资料，卡方检验比较组间性别、基因型、等位基因频率差异，多元Logistic回归分析MMP-9 1562C/T位点基因型的危险因素。 结果： MMP-9 1562C/T位点CC基因型在健康对照组、轻度组、中重度组的检出率分别为31.3%（30/96）、53.5%（53/99）及27.8%（27/97），其分布在健康对照组与轻度组之间、轻度组与中重度之间差异均有统计学意义（χ(2)=9.901 ，P=0.002 ；χ(2)=13.397 ，P< 0.001）。CT基因型在健康对照组、轻度组及中重度组的检出率分别为65.6%（63/96）、45.5%（45/99）及69.1% （67/97），其分布在健康对照组与轻度组之间、轻度组与中重度之间差异均有统计学意义（χ(2)= 8.025，P=0.005；χ(2)=11.159，P<0.001）。等位基因分布在健康对照组与轻度组之间、轻度组与中重度之间差异均有统计学意义（χ(2)=6.270 ，P=0.012 ；χ(2)=8.184 ，P=0.004 ）。多元Logistic回归分析显示，年龄< 35岁是慢性牙周炎的保护因素（OR=0.061，95 %CI=0.035～0.108，P<0.001）；性别为男性是慢性牙周炎的危险因素（OR=2.392，95%CI=1.496~ 3.819，P<0.001）；CT基因型是慢性牙周炎的危险因素（OR= 1.280，95%CI=0.794~ 2.067，P=0.031）。 结论： 新疆墨玉县维吾尔族成人慢性牙周炎的易感性与性别、年龄及MMP-9基因1562C/T位点具有相关性，年龄≥35岁及性别为男性时，慢性牙周炎易感性更高，且随年龄增加慢性牙周炎有加重趋势；CT基因型可能为维吾尔族成人慢性牙周炎的易感因子。.

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