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  • Title: A UPLC-MS/MS method for quantification of 5α-androst-3β,5,6β-triol in human plasma: development, validation and its application in clinical pharmacokinetic study.
    Author: Wang Z, Wang H, Hu P, Jiang J, Zhao Q.
    Journal: Bioanalysis; 2017 Jun; 9(11):873-885. PubMed ID: 28617033.
    Abstract:
    AIM: 5α-androst-3β,5,6β-triol is a novel ischemic stroke drug under clinical development. The objective of this study was to develop and validate a simple ultraperformance liquid chromatography tandem mass spectrometry method for 5α-androst-3β,5,6β-triol in human plasma and its application in clinical pharmacokinetic study. Methodology & results: After being pretreated using an automatized solid-phase extraction procedure, plasma sample was separated on a Waters® Acquity™ BEH C18 column (2.1 × 50 mm id, 1.7 mm) by an Acquity UPLC system and detected by an API 5500 triple quadrupole mass spectrometer, which was validated following international guidelines. CONCLUSION: A simple method was successfully validated over a concentration range of 2.00-500 ng/ml for 5α-androst-3β,5,6β-triol and applied to investigate its plasma pharmacokinetic profiles in healthy Chinese subjects.
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