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  • Title: microRNA‑205 acts as a tumor suppressor and directly targets YAP1 in glioma.
    Author: Ji T, Zhang X, Li W.
    Journal: Mol Med Rep; 2017 Aug; 16(2):1431-1438. PubMed ID: 28627687.
    Abstract:
    Glioma is the most common form of primary malignant tumor that occurs in the central nervous system. The underlying molecular mechanism of the carcinogenesis and progression of glioma remains to be elucidated. It is well‑established that microRNAs (miRs) are associated with the regulation of glioma initiation and progression, and may represent a novel effective therapeutic strategy for the treatment of glioma. In the present study, the expression, roles and molecular mechanisms of miR‑205 in glioma were investigated. The expression levels of miR‑205 in glioma tissues, normal brain tissues, human glioma and normal HEB glial cell lines were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). To explore the functional roles of miR‑205 in glioma cells, a Cell Counting kit 8 assay, and Transwell migration and invasion assays were employed. The molecular mechanisms underlying the roles of miR‑205 in glioma cells were investigated using bioinformatics analysis, a luciferase reporter assay, RT‑qPCR and western blot analysis. The results of the present study demonstrated that miR‑205 expression was markedly low in glioma tissues and cell lines compared with normal brain tissue and a glial cell line. Upregulation of miR‑205 in vitro decreased cell viability, migration and invasion in glioma. Further investigation of the potential molecular mechanism demonstrated that the tumor suppressive functions of miR‑205 in regulating the proliferation, migration and invasion of glioma cells were mediated by a direct target gene, yes associated protein 1 (YAP1). The results of the present study suggested that miR‑205 inhibited glioma growth and metastasis by directly targeting YAP1, and that miR‑205 should be investigated as a novel therapeutic target for anti‑cancer treatment.
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