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  • Title: Intact microtubules are required for rapid turnover of carboxyl-terminal tyrosine of alpha-tubulin in cell cultures.
    Author: Thompson WC, Deanin GG, Gordon MW.
    Journal: Proc Natl Acad Sci U S A; 1979 Mar; 76(3):1318-22. PubMed ID: 286314.
    Abstract:
    In cultured muscle cells the carboxyl-terminal tyrosine of alpha-tubulin was shown to exchange rapidly with free tyrosine. The rapid turnover of this residue was dependent upon the presence of intact microtubules. Half-life determinations were made by two methods: (i) the cells were pulse-labeled in hypertonic medium, in which the major tyrosine incorporation was post-translational, and then chased with isotonic medium; and (ii) the cells were pulsed and chased in isotonic medium, and the post-translational component of the radioactivity of purified alpha-tubulin was calculated. Both methods yielded a half-life of 37 min or less for the terminal tyrosine residue, whereas the half-life of tubulin itself was shown to be greater than 48 hr.
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