These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France.
    Author: Moura A, Tourdjman M, Leclercq A, Hamelin E, Laurent E, Fredriksen N, Van Cauteren D, Bracq-Dieye H, Thouvenot P, Vales G, Tessaud-Rita N, Maury MM, Alexandru A, Criscuolo A, Quevillon E, Donguy MP, Enouf V, de Valk H, Brisse S, Lecuit M.
    Journal: Emerg Infect Dis; 2017 Sep; 23(9):1462-1470. PubMed ID: 28643628.
    Abstract:
    During 2015-2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p<0.001). cgMLST discriminated unrelated isolates that shared identical PFGE profiles and phylogenetically closely related isolates with distinct PFGE profiles. This procedure also refined epidemiologic investigations to include only phylogenetically closely related isolates, improved source identification, and facilitated epidemiologic investigations, enabling identification of more outbreaks at earlier stages. WGS-based typing should replace PFGE as the primary typing method for L. monocytogenes.
    [Abstract] [Full Text] [Related] [New Search]