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  • Title: Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.
    Author: Thompson RE, Hewitt CR, Piper DJ, Hansen WP, Rubin RH, Tolkoff-Rubin NE, Barrett MC, Nelles MJ.
    Journal: Clin Chem; 1985 Nov; 31(11):1833-7. PubMed ID: 2865018.
    Abstract:
    This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.
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