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  • Title: Diamide blocks H+ conductance in mitochondrial H+-ATPase by oxidizing FB dithiol.
    Author: Huang Y, Pringle MJ, Sanadi DR.
    Journal: FEBS Lett; 1985 Nov 11; 192(1):83-7. PubMed ID: 2865171.
    Abstract:
    Effects of diamide on proton conductance of electron transport particles (ETPH), purified H+-ATPase (F1-F0), F0 of the H+-ATPase from beef heart mitochondria and binding of cadmium (109Cd) to the H+-ATPase have been examined in the present paper. When ETPH and purified H+-ATPase are treated with 1 mM diamide, ATP-dependent generation of membrane potential, monitored by the absorbance change produced by the redistribution of oxonol VI, is consistently inhibited. Diamide also blocks passive H+ conductance driven by a K+ diffusion potential in the membrane sector, F0, of H+-ATPase. Furthermore, diamide treatment drastically reduces the binding of 109Cd2+ to H+-ATPase, showing competition for the FB dithiol group.
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