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  • Title: ANF stimulation of detergent-dispersed particulate guanylate cyclase from bovine adrenal cortex.
    Author: Tremblay J, Gerzer R, Pang SC, Cantin M, Genest J, Hamet P.
    Journal: FEBS Lett; 1986 Jan 06; 194(2):210-4. PubMed ID: 2866981.
    Abstract:
    Particulate guanylate cyclase from bovine adrenal cortex can be stimulated by ANF. A 2-fold stimulation of the enzyme was obtained with 100 nM ANF and a half-maximal stimulation, with a 5 nM dose. The stimulation by ANF persisted for at least 30 min. Various detergents, such as Triton X-100, Lubrol PX, cholate, CHAPS, digitonin and zwittergent, stimulated several-fold the activity of particulate guanylate cyclase. However, only Triton X-100 dispersed particulate guanylate cyclase without affecting its response to ANF. The dose-response curve of ANF stimulation of the particulate and the Triton X-100 dispersed enzyme was similar. The dispersion of a fully responsive guanylate cyclase to ANF will help us to uncover the type of interactions between guanylate cyclase and ANF. It will also be used as a first step for the purification of an ANF-sensitive particulate guanylate cyclase.
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