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  • Title: Isolation and propagation of hepatitis A virus in hepatoma cell cultures.
    Author: Friedman-Alvermann B, Müller D, Spies K.
    Journal: Acta Virol; 1985 Dec; 29(6):482-6. PubMed ID: 2869659.
    Abstract:
    Hepatitis A virus (HAV) was isolated directly from human faeces in PLC/PRF/5 cells. In the first passage cell-bound and supernatant viruses were found by immune electron microscopy and by enzymeimmunoassay. Serial passaging of HAV in PLC/PRF/5 cells resulted in its adaptation to the cell line and in reduction of the incubation time. HAV was still detectable after 10 cell passages. Cell-bound as well as supernatant HAV were employed as antigens in anti HAV IgM-enzymeimmunoassay.
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