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  • Title: Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China.
    Author: Dong YP, Wang W, Jiang T, Xu J, Han CH, Yan SF, Fanning S, Li Y, Ma XC, Zhang D, Zhao Y, Zeng B, Li FQ.
    Journal: Biomed Environ Sci; 2017 Jun; 30(6):460-464. PubMed ID: 28705271.
    Abstract:
    Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.
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