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  • Title: Desaturation and chain elongation of essential fatty acids in isolated liver cells from rat and rainbow trout.
    Author: Hagve TA, Christophersen BO, Dannevig BH.
    Journal: Lipids; 1986 Mar; 21(3):202-5. PubMed ID: 2871473.
    Abstract:
    Isolated hepatocytes from rainbow trout and rat were incubated with 14C-labeled linoleic acid, linolenic acid, dihomogammalinolenic acid or eicosapentaenoic acid. The most striking difference in the desaturase activity was the lower level of delta 5 desaturase in trout than in rat. No delta 4 desaturation of 22:4(n-6) to 22:5(n-6) was observed in either of the two species, while the conversion of 22:5(n-3) to 22:6(n-3) was significant in both groups and highest in rainbow trout. The chain-elongating activity was remarkably similar in the two species, except for the "dead-end" elongation which was distinctly more important in fish.
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