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  • Title: A two-step bioprocessing strategy in pentonic acids production from lignocellulosic pre-hydrolysate.
    Author: Zhou X, Huang L, Xu Y, Yu S.
    Journal: Bioprocess Biosyst Eng; 2017 Nov; 40(11):1581-1587. PubMed ID: 28721445.
    Abstract:
    Efficient utilization (over needless disposal) of biorefinery pre-hydrolysate is an economically relevant practice for improving biorefinery financial prospects. The liquid fraction obtained after acid hydrolysis pretreatment of lignocellulosic biomass, called pre-hydrolysate, are predominantly comprised of hemicellulosic carbohydrates. Using a two-step bioprocess, the hexoses were selectively fermented to ethanol by S. cerevisiae to clear the path for Gluconobacter oxydans transformation of pentoses to a high purity pentonic acids solution. Finally, approximately 180 g pentonic acids and 19 g ethanol could be produced starting from pre-hydrolysate produced from 1 kg corn stover. The results demonstrate execution of our objective to prove this bioconversion method for producing high purity pentonic acids starting from crude lignocellulosic pre-hydrolysate, a wastefully disregarded biorefinery process stream.
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