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  • Title: Purification and properties of an induced beta-D-glucosidase from stachybotrys atra.
    Author: de Gussem RL, Aerts GM, Claeyssens M, de Bruyne CK.
    Journal: Biochim Biophys Acta; 1978 Jul 07; 525(1):142-53. PubMed ID: 28763.
    Abstract:
    We have purified an induced beta-D-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) from Stachybotrys atra to apparent homogeneity. The induced enzyme was clearly different from the constitutive beta-D-glucosidase. The molecular weight was 65 500-69 000, the pH optimum was at 6.7 and the isoelectric point at 4.8. Carbohydrate content (related to glucose) was 14.4%. The enzyme showed beta-D-glucosidase, beta-D-xylosidase and beta-D-thioglucosidase activity. These three activities sppear to be due to the same enzyme. The enzyme was strongly inhibited by D-glucono-(1 leads to 5)-lactone and nojirimycin and was very sensitive to sulfhydryl reagents.
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