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  • Title: [Evaluation of the viability of BEAS-2B cells exposed to gasoline engine exhaust with different particle sizes by air-liquid interface].
    Author: Yu T, Zhang XY, Wang ZX, Li B, Zheng YX, Bin P.
    Journal: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2017 Jun 20; 35(6):414-421. PubMed ID: 28780815.
    Abstract:
    Objective: To evaluate the viability of gasoline engine exhaust (GEE) with different particle sizes on human lung cell line BEAS-2B in vitro by air-liquid interface (ALI) . Methods: GEE were collected with a Tedlar bag and their particulate matter (PM) number, surface and mass concentration in three kind of GEE (filtered automobile exhaust, non-filtered automobile exhaust and motorcycle exhaust without three-way catalytic converter) were measured by two type of particle size spectrometer including TSI-3321 and SMPS-3938. Five groups were included, which divided into blank control group, clean air group, filtered automobile exhaust group, non-filtered automobile exhaust group and motorcycle exhaust without three-way catalytic converter group. Except the blank control group, BEAS-2B cells, cultured on the surface of Transwells, were treated with clean air or GEE by ALI method at a flow rate of 25 ml/min, 37 ℃ for 60 min in vitro. CCK-8 cytotoxicity test kit was used to determine the cell relative viability of BEAS-2B cells. Results: In the filtered automobile exhaust, non-filtered automobile exhaust and motorcycle exhaust without three-way catalytic converter, high concentrations of fine particles can be detected, but the coarse particles only accounted for a small proportion, and the sequence of PM concentration was motorcycle exhaust without three-way catalytic converter group> non-filtered automobile exhaust group> filtered automobile exhaust group (P<0.001) . Compared with the clean air group, the cell relative viability in the 3 GEE-exposed groups were significantly lower (P<0.001) . Among the comparisons of GEE exposure groups with different particle size spectra, the sequence of the cell relative viability was filtered automobile exhaust group >non-filtered automobile exhaust group> motorcycle exhaust without three-way catalytic converter group (P<0.001) . When took the clean air control group as a reference, the mean of the cell relative viability in the filtered automobile exhaust group, non-filtered automobile exhaust group and motorcycle exhaust without three-way catalytic converter group, was decreased by 26.34%, 36.00% and 49.59%, respectively. Conclusion: GEE with different particle size spectra could induce different levels of toxic effects to the human lung cells BEAS-2B by ALI. After lowering the concentration of particles in the GEE and using the three-way catalytic converter could obviously improve the survival rate of lung cells. 目的： 采用气-液界面（ALI）体外细胞染毒技术，评价不同粒径谱汽油发动机尾气（GEE）对人肺BEAS-2B细胞存活率的影响。 方法： 用Tedlar集气袋收集GEE，并通过TSI-3321和SMPS-3938两种粒径谱仪分析过滤后的汽车尾气组、汽车尾气组和非三元催化配置的摩托车尾气组的颗粒物个数、表面积和质量浓度，同时设置空白对照组和洁净空气组。除了空白对照组，其余4组采用ALI体外细胞染毒技术，在染毒流量为25 ml/min，37 ℃水浴条件下，对生长在Transwell插件多孔膜上的BEAS-2B细胞持续染毒60 min。通过CCK-8细胞毒性检测试剂盒测定细胞的相对存活率。 结果： 过滤后的汽车尾气组、汽车尾气组和摩托车尾气组均可检出较高浓度的细颗粒物，但粗颗粒物很少，且呈摩托车尾气组>汽车尾气组>过滤后的汽车尾气组的趋势（P<0.001）。与洁净空气组比较，过滤后的汽车尾气组、汽车尾气组和摩托车尾气组的细胞相对存活率均明显降低，差异有统计学意义（P<0.001）。各GEE暴露组之间的比较显示，细胞相对存活率呈过滤后的汽车尾气组>汽车尾气组>摩托车尾气组的趋势（P<0.001）；以洁净空气组为参考，过滤后的汽车尾气组、汽车尾气组、非三元催化配置的摩托车尾气组的细胞相对存活率均数依次降低了26.34%、36.00%和49.59%。 结论： 人肺BEAS-2B细胞ALI暴露于不同粒径谱的GEE可产生不同程度的毒性作用，降低GEE颗粒物浓度以及配置三元催化装置可明显改善肺细胞的存活率。.
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