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  • Title: The titration of clostridial toxoids and antisera in cell culture.
    Author: Knight PA, Burnett C, Whitaker AM, Queminet J.
    Journal: Dev Biol Stand; 1986; 64():129-36. PubMed ID: 2878845.
    Abstract:
    The practicability of cell culture titration methods for antigens and antitoxins derived from Clostridium perfringens, Cl. septicum and Cl. novyi was investigated. Toxin neutralization could not be demonstrated with Cl. perfringens filtrates but assays for antitoxin based on the use of cultures of Vero cells were practicable for Cl. novyi and Cl. septicum. In the case of the latter, the precision and reproducibility of the test was sufficient for quality control purposes.
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