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  • Title: Label-Free Direct Electrical Detection of a Histidine-Rich Protein with Sub-Femtomolar Sensitivity using an Organic Field-Effect Transistor.
    Author: Minamiki T, Sasaki Y, Tokito S, Minami T.
    Journal: ChemistryOpen; 2017 Aug; 6(4):472-475. PubMed ID: 28794937.
    Abstract:
    There is a growing interest in achieving sensor systems to enable on-site testing of biomarkers. Herein, a new strategy for highly sensitive protein detection at sub-femtomolar levels without any labelling has been demonstrated by using an organic field-effect transistor (OFET). An artificial histidine-rich protein receptor (NiII-nitrilotriacetic acid complex, NiII-nta) functionalizes a detection portion (i.e. an extended-gate electrode) of the fabricated OFET device. The OFET responds electrically and selectively to a target analyte (bovine serum albumin), meaning that the binding processes at the NiII-nta on the extended-gate electrode for the analyte affect the field-effect properties of the device. Our results demonstrate that the combination of the OFET with the artificial receptor is an ideal approach for label-free and immune-free protein detection.
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