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  • Title: Carbon flux through citric acid cycle pathways in perfused heart by 13C NMR spectroscopy.
    Author: Malloy CR, Sherry AD, Jeffrey FM.
    Journal: FEBS Lett; 1987 Feb 09; 212(1):58-62. PubMed ID: 2879743.
    Abstract:
    Mathematical models of the TCA cycle derived previously for 14C tracer studies have been extended to 13C NMR to measure the 13C fractional enrichment of [2-13C]acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of 13C into the glutamate carbons. Hearts perfused with [2-13C]acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with [2-13C]acetate. In each case, the amount of [2-13C]acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated.
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