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  • Title: Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA.
    Author: Mor SK, Phelps NBD, Ng TFF, Subramaniam K, Primus A, Armien AG, McCann R, Puzach C, Waltzek TB, Goyal SM.
    Journal: Arch Virol; 2017 Dec; 162(12):3619-3627. PubMed ID: 28815386.
    Abstract:
    During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".
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