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  • Title: Estimation of Photorespiratory Fluxes by Gas Exchange.
    Author: Busch FA, Deans RM, Holloway-Phillips MM.
    Journal: Methods Mol Biol; 2017; 1653():1-15. PubMed ID: 28822122.
    Abstract:
    Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78-90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its 12CO2 and 13CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using 13CO2.
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