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Title: [Cytokine profiles of CD4(+) T memory cells in asthma and their relationship with asthma severity].
Author: Du CL, Xu K, Min ZH, Li DD, Yuan HL, Liu C, Chen ZH.
Journal: Zhonghua Yi Xue Za Zhi; 2017 Aug 08; 97(30):2333-2337. PubMed ID: 28822449.
Abstract:
Objective: To study the phenotype of memory CD4(+) T cells in peripheral blood of asthmatics and its relationship with asthma severity. Methods: From Dec 2014 to Aug 2015, thirty-three asthmatics, twenty-six chronic obstructive pulmonary disease (COPD) patients and twenty-two healthy volunteers were enrolled in Respiratory Clinics of Zhongshan Hospital, Fudan University. Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood. Cell surface markers (CD45RO, CRTH2, CD62L, and CCR7) and intracellular protein[interleukin (IL)-5, IL-17, interferon (IFN)-γ]staining was performed using flow-cytometric techniques. CD4(+) T cells were cultured under neutralization and then Th2, Th2+ Lipopolysaccharide (LPS), Th2+ Home dust mite (HDM) conditions for 6 days and then intracellular proteins were analyzed using flow cytometry. Correlation analysis between memory CD4(+) T cells, asthma severity and drug consumption were performed. Results: The percentage of memory CD4(+) T (CD4(+) Tm) cells in circulating white blood cells was higher in asthmatics, than that in healthy subjects (48.0%±5.7% vs 32.0%±4.1%, P<0.05). The cytokine profiles of CD4(+) Tm cells in asthma patients were substantially different from those of COPD and healthy subjects, with increased IL-5 and IL-17 production. For COPD patients, the predominant cytokines were IFN-γ instead. IL-17-producing CD4(+) Tm cells were associated with the severity of disease and the level of medication consumption in asthma patients (R(2)=0.829 6, P<0.05). Conclusions: The cytokine profile is IL-5 and IL-17 predominant in memory CD4(+) T cells from asthmatics. The amount of IL-17(+) CD4(+) memory T cells is positively correlated with asthma severity. 目的： 探讨外周血CD4(＋)记忆T细胞的表型及其与支气管哮喘(简称哮喘)严重程度的相关性。 方法： 2014年12月至2015年8月在复旦大学附属医院中山医院呼吸科招募哮喘患者32例(哮喘组)，并将慢性阻塞性肺病(简称慢阻肺)患者26例(慢阻肺组)及健康对照22名(健康对照组)作为对照。将受试者外周血分离的单个核细胞(PBMCs)进行细胞表面标记(CD45RO、CRTH2、CD62L、CCR7)和细胞内因子染色，如白细胞介素(IL)－5、IL－17、γ－干扰素(IFN－γ)。CD4(＋)T细胞在中性、Th2、Th2＋脂多糖(LPS)、Th2＋屋尘螨抗原(HDM)条件下培养6 d，刺激细胞后用流式细胞仪检测胞内细胞因子水平。CD4(＋)记忆T细胞数量与哮喘严重程度及药物使用级别之间进行相关分析。 结果： 哮喘组外周血中CD4(＋)记忆T细胞数量显著高于健康对照组(48.0%±5.7%比32.0%±4.1%，P<0.05)，其分泌的细胞因子与慢阻肺组和健康对照组不同，哮喘组以IL－5、IL－17高分泌为主，而慢阻肺组以IFN－γ高分泌为主。IL－17(＋)CD4(＋)记忆T细胞数量与哮喘严重程度相关。IL－17(＋)CD4(＋)记忆T细胞数量与药物使用级别呈正相关(R(2)＝0.829 6，P<0.05)。 结论： 哮喘组CD4(＋)记忆T细胞分泌的细胞因子以IL－5、IL－17为主，且IL－17(＋)CD4(＋)记忆T细胞数量与哮喘严重程度呈正相关。.

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