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  • Title: Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC).
    Author: Huang T, He J.
    Journal: Methods Mol Biol; 2017; 1660():191-199. PubMed ID: 28828657.
    Abstract:
    Extracellular vesicles (EVs) have recently attracted substantial attention due to the potential diagnostic and therapeutic relevance. Although a variety of techniques have been used to isolate and analyze EVs, it is still far away from satisfaction. Size-exclusion chromatography (SEC), which separates subjects by size, has been widely applied in protein purification and analysis. The purpose of this chapter is to show the applications of size-exclusion high-performance liquid chromatography (HPLC) as methods for EV characterization of impurities or contaminants of small size, and thus for quality assay for the purity of the samples of EVs.
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