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  • Title: Human-immunodeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay.
    Author: Borkowsky W, Krasinski K, Paul D, Moore T, Bebenroth D, Chandwani S.
    Journal: Lancet; 1987 May 23; 1(8543):1168-71. PubMed ID: 2883490.
    Abstract:
    Of 85 children with human-immuno-deficiency-virus (HIV) infection based on clinical (opportunistic infection), epidemiological (mother a drug addict or known to be HIV infected), and immunological (helper-T-cell deficiency and impaired proliferative response to pokeweed mitogen) features, 9 were found to lack antibody to HIV as measured by a commercial enzyme-linked immunoassay (ELISA). All 9 children had detectable levels of HIV antigen in simultaneous plasma specimens, measured by a sensitive antigen-capture ELISA. The use of the western blot assay and an ELISA with recombinant HIV antigens was able to identify HIV infection in 4 of the 9 children.
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