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  • Title: Identification and molecular weight of SP1 synthesized from mRNA of human placenta in a wheat germ cell-free system.
    Author: Bocco JL, Actis A, Flury A, Patrito LC.
    Journal: Mol Biol Rep; 1987; 12(1):55-9. PubMed ID: 2888015.
    Abstract:
    Poly (A+)-mRNA obtained from human term placenta using guanidine HCl and oligo (dT) cellulose chromatography was translated in a wheat germ cell-free system. SDS-polyacrylamide gel electrophoresis analysis of the translation products revealed the presence of several polypeptides with molecular weights ranging from 10 KD to 70 KD. A single protein band representing around 1% of the total radioactive proteins synthesized in the presence of 2.5 micrograms of mRNA was isolated by immunoprecipitation, using specific antiserum against either the native 'Pregnancy-specific beta 1-glycoprotein' or a reduced and carboxymethylated derivative. The molecular weight of 31-2 KD of this translation product corresponding to the nonprocessed precursor could account for the 43 KD value assigned to the protein purified form human pregnant serum.
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