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  • Title: [Importance of urinary enzymes as a marker of post-ischemic proximal tubular involvement in rat].
    Author: Catroux P, Dorian C, Harpey C, Cambar J.
    Journal: C R Seances Soc Biol Fil; 1987; 181(3):307-13. PubMed ID: 2888519.
    Abstract:
    We have studied in rats the influence of renal ischemia on urinary excretion of three brush border membrane enzymes (gamma glutamyl transferase, alkaline phosphatase and leucine aminopeptidase) and of a lysosomal one (N-acetyl-B-D-glucosaminidase). Urines were collected over 24 hours periods during three days before and after a 45 minutes renal artery clamping. Urinary GGT, PAL and LAP excretion were significantly increased on the first day after renal ischemia, but returns to normal values on the second day. Urinary NAG activity increases on the first day, but contrary to the latter enzymes, reached to normal values only on the third day. Enzymuria seems to be a useful marker of tubular injury occurring after a temporary renal ischemia in the rat.
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