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  • Title: Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2.
    Author: Tandeau de Marsac N, de la Torre F, Szulmajster J.
    Journal: Mol Gen Genet; 1987 Sep; 209(2):396-8. PubMed ID: 2890082.
    Abstract:
    A 3.6 kb HindIII DNA fragment from Bacillus sphaericus 1593M was cloned and expressed in Escherichia coli and B. subtilis using pHV33 as shuttle vector and in the cyanobacterium Anacystis nidulans R2 with pUC303 as shuttle vector. The level of toxin activity of the respective recombinant plasmids pGsp04 and pGsp12 against Culex mosquito larvae was found to be the same in Escherichia coli and in the cyanobacterium.
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