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  • Title: An HPLC assay procedure of sensitivity and stability for measurement of acetylcholine and choline in neuronal tissue.
    Author: Barnes NM, Costall B, Fell AF, Naylor RJ.
    Journal: J Pharm Pharmacol; 1987 Sep; 39(9):727-31. PubMed ID: 2890738.
    Abstract:
    A method is presented for the sensitive and specific determination of acetylcholine and choline in neuronal tissue. The method is based on the separation of acetylcholine and choline by reversed-phase HPLC, passing the eluent into a post-column reactor containing choline oxidase and acetylcholinesterase covalently bound to vinyl sulphone bonded onto a hydroxyethyl methacrylate support, and electrochemical detection of the hydrogen peroxide formed. The limit of detection of the procedure is 1 pmol for acetylcholine and 500 fmol for choline. The excellent baseline stability of the method ensures the rapid and reliable processing of a large number of samples.
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