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  • Title: [Effects of arnebia root oil on wound healing of rats with full-thickness skin defect and the related mechanism].
    Author: Shen JY, Ma Q, Yang ZB, Gong JJ, Wu YS.
    Journal: Zhonghua Shao Shang Za Zhi; 2017 Sep 20; 33(9):562-567. PubMed ID: 28926878.
    Abstract:
    Objective: To observe the effects of arnebia root oil on wound healing of rats with full-thickness skin defect, and to explore the related mechanism. Methods: Eighty SD rats were divided into arnebia root oil group and control group according to the random number table, with 40 rats in each group, then full-thickness skin wounds with area of 3 cm×3 cm were inflicted on the back of each rat. Wounds of rats in arnebia root oil group and control group were treated with sterile medical gauze and bandage package infiltrated with arnebia root oil gauze or Vaseline gauze, respectively, with dressing change of once every two days. On post injury day (PID) 3, 7, 14, and 21, 10 rats in each group were sacrificed respectively for general observation and calculation of wound healing rate. The tissue samples of unhealed wound were collected for observation of histomorphological change with HE staining, observation of expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) with immunohistochemical staining, and determination of mRNA expressions of VEGF and bFGF with real time fluorescent quantitive reverse transcription polymerase chain reaction. Data were processed with analysis of variance of factorial design, t test, and Bonferroni correction. Results: (1) On PID 3, there were a few secretions in wounds of rats in the two groups. On PID 7, there were fewer secretions and more granulation tissue in wounds of rats in arnebia root oil group, while there were more secretions and less granulation tissue in wounds of rats in control group. On PID 14, most of the wounds of rats in arnebia root oil group were healed and there was much red granulation tissue in unhealed wounds, while part of wounds of rats in control group was healed and there were a few secretions and less granulation tissue in unhealed wounds. On PID 21, wounds of rats in arnebia root oil group were basically healed, while there were still some unhealed wounds of rats in control group. (2) On PID 3 and 7, the wound healing rates of rats in arnebia root oil group were (39±5)% and (46±4)% respectively, which were close to (34±3)% and (44±4)% of rats in control group (with t values respectively 0.807 and 0.481, P values above 0.05). On PID 14 and 21, the wound healing rates of rats in arnebia root oil group were (76±4)% and (90±3)% respectively, which were significantly higher than (60±6)% and (73±5)% of rats in control group (with t values respectively 2.308 and 3.072, P<0.05 or P<0.01). (3) On PID 3, 7, and 14, granulation tissue, fibroblasts, and nascent capillaries in unhealed wound tissue of rats in the two groups both gradually increased, and more ranulation tissue, fibroblasts, and nascent capillaries were seen in unhealed wound tissue of rats in arnebia root oil group. On PID 21, granulation tissue, fibroblasts, and nascent capillaries in unhealed wound tissue of rats in the two groups both gradually decreased. (4) On PID 3, 7, and 14, the numbers of VEGF positive cells and bFGF positive cells in unhealed wound tissue of rats in the two groups both gradually increased; there were more VEGF positive cells and bFGF positive cells in unhealed wound tissue of rats in arnebia root oil group than those in control group. On PID 21, positive expressions of VEGF and bFGF both decreased in unhealed wound tissue of rats in the two groups. (5) On PID 3, 7, and 14, mRNA expressions of VEGF in unhealed wound tissue of rats in arnebia root oil group were higher than those of control group (with t values from 2.967 to 4.173, P values below 0.01). On PID 21, mRNA expression of VEGF in unhealed wound tissue of rats in arnebia root oil group was lower than that of control group (t=-4.786, P<0.001). From PID 3 to 21, mRNA expressions of bFGF in unhealed wound tissue of rats in arnebia root oil group were higher than those of control group (with t values from 2.326 to 4.702, P<0.05 or P<0.01). Conclusions: Arnebia root oil can promote wound healing of rats with full-thickness skin defect, which may relate to increasing expressions of VEGF and bFGF. 目的： 观察紫草油剂对全层皮肤缺损大鼠创面愈合的影响并探讨相关机制。 方法： 取80只SD大鼠，按随机数字表法分为紫草油剂组和对照组，每组40只，于背部制作3 cm×3 cm全层皮肤缺损创面。紫草油剂组、对照组大鼠创面分别以紫草油剂浸润的纱布、凡士林纱布和无菌医用纱布打包包扎，均每2天换药1次。伤后3、7、14、21 d，每组分别取10只大鼠处死后，行创面大体观察并计算创面愈合率，取创面未愈合组织行HE染色观察组织形态学变化、免疫组织化学染色观察血管内皮生长因子(VEGF)和bFGF表达情况、实时荧光定量RT-PCR检测VEGF和bFGF的mRNA表达。对数据行析因设计方差分析、t检验及Bonferroni校正。 结果： (1)伤后3 d，2组大鼠创面均有少量分泌物。伤后7 d，紫草油剂组大鼠创面分泌物减少，肉芽组织较多；而对照组大鼠创面仍有较多分泌物，肉芽组织较少。伤后14 d，紫草油剂组大鼠创面大部分已愈合，未愈合创面肉芽组织多且鲜红；对照组大鼠部分创面愈合，未愈合创面肉芽组织少，仍有分泌物。伤后21 d，紫草油剂组大鼠创面基本愈合，对照组大鼠创面仍有部分未愈合。(2)伤后3、7 d，紫草油剂组大鼠创面愈合率分别为(39±5)%和(46±4)%，与对照组的(34±3)%和(44±4)%相近(t值分别为0.807和0.481，P值均大于0.05)；伤后14、21 d，紫草油剂组大鼠创面愈合率分别为(76±4)%和(90±3)%，明显高于对照组的(60±6)%和(73±5)%(t值分别为2.308和3.072，P<0.05或P<0.01)。(3)伤后3、7、14 d，2组大鼠创面未愈合组织中肉芽组织、Fb和新生微血管均逐渐增多，且紫草油剂组大鼠创面未愈合组织中肉芽组织、Fb和新生微血管均较对照组多。伤后21 d，2组大鼠创面未愈合组织中肉芽组织、Fb和新生微血管逐渐减少。(4)伤后3、7、14 d，2组大鼠创面未愈合组织中VEGF阳性细胞和bFGF阳性细胞均逐渐增多，且紫草油剂组大鼠创面未愈合组织中VEGF阳性细胞和bFGF阳性细胞均较对照组多。伤后21 d, 2组大鼠创面未愈合组织中VEGF和bFGF阳性表达均减弱。(5)紫草油剂组大鼠伤后3、7、14 d，创面未愈合组织中VEGF的mRNA表达量明显高于对照组(t值为2.967～4.173，P值均小于0.01)；伤后21 d，创面未愈合组织中VEGF的mRNA表达量明显低于对照组(t＝－4.786，P<0.001)。紫草油剂组大鼠伤后3～21 d创面未愈合组织中bFGF的mRNA表达量均明显高于对照组(t值为2.326～4.702，P<0.05或P<0.01)。 结论： 紫草油剂外用可促进全层皮肤缺损大鼠创面愈合，可能与其促进创面组织高表达VEGF和bFGF相关。.
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