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  • Title: The use of DNA probes identifying restriction-fragment-length polymorphisms to examine the Mycobacterium avium complex.
    Author: McFadden JJ, Butcher PD, Thompson J, Chiodini R, Hermon-Taylor J.
    Journal: Mol Microbiol; 1987 Nov; 1(3):283-91. PubMed ID: 2896288.
    Abstract:
    DNA probes were used to identify restriction-fragment-length polymorphisms (RFLPs) in DNA samples, demonstrating that the Mycobacterium avium complex could be clearly divided into M. avium and Mycobacterium intracellulare strains. Less than 2% DNA base substitution was found between M. avium strains, whereas the M. intracellulare strains had greater than 15% base substitution. The Johne's disease bacillus, Mycobacterium paratuberculosis (American type strain), was found to be distinguishable from the M. avium complex serotypes examined. Strain 18 was found to be identical to M. avium. The rat leprosy bacillus, Mycobacterium lepraemurium, was found to be very closely related, but not identical, to M. avium.
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