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  • Title: Dynamic multicolor protein labeling in living cells.
    Author: Li C, Plamont MA, Sladitschek HL, Rodrigues V, Aujard I, Neveu P, Le Saux T, Jullien L, Gautier A.
    Journal: Chem Sci; 2017 Aug 01; 8(8):5598-5605. PubMed ID: 28970939.
    Abstract:
    Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST's reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging.
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