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  • Title: The poly(A)-binding protein facilitates in vitro translation of poly(A)-rich mRNA.
    Author: Grossi de Sa MF, Standart N, Martins de Sa C, Akhayat O, Huesca M, Scherrer K.
    Journal: Eur J Biochem; 1988 Oct 01; 176(3):521-6. PubMed ID: 2901956.
    Abstract:
    To investigate the role of the 73-kDa poly(A)-binding protein in protein synthesis, the effect of the addition of homo-polyribonucleotides on the translation of polyadenylated and non-adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleotide in inhibiting duck-globin mRNA translation, whereas it had no effect on the translation of polyribosomal duck-globin mRNP, or on the endogenous synthesis of the rabbit reticulocyte lysate. The translation of poly(A)-free mRNA was not affected by the addition of poly(A). Furthermore, we found that the inhibiting effect of poly(A) can be reversed by addition of purified poly(A)-binding protein. It is thus likely that the 73-kDa poly(A)-binding protein is an essential factor necessary for poly(A)-rich mRNA translation.
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