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  • Title: A specific HPLC method for determination of beta-blockers topically used in ophthalmological diseases.
    Author: Tracqui A, Kintz P, Himber J, Lugnier AA, Mangin P.
    Journal: Forensic Sci Int; 1988; 38(1-2):37-41. PubMed ID: 2903842.
    Abstract:
    A HPLC method is presented for the identification and quantification in plasma and urine of beta-adrenergic receptor antagonists (betaxolol, carteolol, metipranolol, and timolol) commonly prescribed in ophthalmology. An extraction method is described using pindolol as an internal standard. An RSIL 10 micron column was used. The lower detection limits of the beta-blockers were found to be 4-27 ng/ml. This method is simple, rapid and sensitive; moreover, it allows the determination of 8 other beta-blockers.
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