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Title: [Cytokine analysis of aqueous humor in AIDS patients].
Author: Kong WJ, Guo CG, Xie LY, Wei WB, Dong HW, Chen C, Du KF.
Journal: Zhonghua Yan Ke Za Zhi; 2017 Oct 11; 53(10):746-752. PubMed ID: 29050187.
Abstract:
Objective: To explore the Cytokine of aqueous humor in AIDS patients with different eye diseases. Methods: A case-control study including 38 patients who was diagnosed as acquired immunodeficiency syndrome(AIDS) at Infectious Center and Department of Ophthalmology in Beijing You'an Hospital, Capital Medical University. And 16 cases of 38 patients were cytomegalovirus retinitis (CMVR), 14 cases were HIV-related retinal disease, and 8 cases presented as normal. A series of tests were performed including vision acuity check, intraocular pressure, fundus photography, blood cell count of CD4+ T lymphocyte and the content of cytokines in aqueous humor by Luminex 200(TM) liquid chip analyzer. Intraocular pressure was analyzed by one-way ANOVA, visual acuity, CD4(+) T lymphocyte count and cytokines were analyzed by Kruskal-Wallis test, the differences between the two groups were compared by Mann-Whitney U test corrected with Bonferroni. Results: Of the 38 AIDS patients [mean age (33.7±10.0) year], 37 were males and 1 was female. The mean visual acuity of 38 patients is logMAR 1.00(0.000, 1.000). The difference of visual acuity among three groups was statistically significant (χ(2)=9.963, P=0.007). The visual acuity in CMVR group was significantly higher than that in HIV-related retinal disease group and AIDS-normal eye group. There was no statistically significant difference of intraocular pressure among three groups (F=0.830, P<0.05). The mean CD4+ T lymphocyte count was 58(4, 550)/μl and the difference was statistically significant among three groups(χ(2)=6.106, P=0.047). The CD4+ T lymphocyte count in AIDS-normal eye group was significantly higher than that in CMVR group and HIV-related retinal disease group. There was no statistically significant difference in CD4+ T lymphocyte count between HIV-related retinal disease group and CMVR group. The following cytokines in CMVR group were significantly higher than the other two groups with statistically significant differences: MIP-1b, IL-6, TNF-α, VEGF, IL-8, MCP-1, IP-10. Conclusions: In patients who was diagnosed as AIDS, cytomegalovirus retinitis would damage vision acuity seriously. The elevated cytokines' level in aqueous humor such as MIP-1b, IL-6, TNF-α, VEGF, IL-8, MCP-1, IP-10, compared with HIV-related retinal disease and AIDS-normal eye cases, may be related to the activity of Th1 cell and monocyte-macrophages system including chemokines, inflammatory factors, and vascular endothelial factors. (Chin J Ophthalmol, 2017, 53: 746-752). 目的： 探讨不同眼部表现的获得性免疫缺陷综合征（AIDS）患者房水中细胞因子的含量变化情况。 方法： 病例对照研究。选取2013年12月至2015年7月在首都医科大学附属北京佑安医院感染中心及眼科确诊的AIDS患者38例，男性37例，女性1例，平均（33.7±10.0）岁。按眼部表现分为巨细胞病毒视网膜炎（CMVR）组（16例）、人类免疫缺陷病毒（HIV）视网膜病变组（14例）、眼部正常组（8例），分别进行视力（logMAR）、眼压、眼底照相眼科常规检查，同时检测血液中CD4+T细胞数目，分别对3个组进行前房穿刺抽液，采用Luminex 200液相芯片分析仪测定各组房水中细胞因子的含量。眼压比较采用单因素方差分析。视力、CD4+T细胞数、细胞因子含量采用Kruskal-Wallis检验，Mann-Whitney U检验比较两组间差异,组间比较行Bonferroni校正。 结果： 38例患者视力为1.000（0.000，1.000），3个组视力之间的差别具有统计学意义（χ(2)=9.963，ν=2，P=0.007）,CMVR组明显高于视网膜病变组和眼部正常组。3个组患者的眼压为（13.82±3.91）mmHg（1 mmHg=0.133 kPa），3个组眼压的差异无统计学意义（F=0.830，P=0.444）。3个组CD4+T细胞数目58（4，550）个/μl，组间比较差异有统计学意义（χ(2)=6.106，ν=2，P=0.047），眼部正常组的CD4+T细胞数明显高于CMVR组和视网膜病变组，CMVR组和视网膜病变组CD4+T细胞数差异无统计学意义。巨噬细胞炎性蛋白1b（MIP-1b）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、血管内皮生长因子（VEGF）、白细胞介素8（IL-8）、单核细胞趋化蛋白1（MCP-1）、干扰素诱导蛋白10（IP-10）的含量在3个组之间的差异有统计学意义，且CMVR组上述7个因子含量明显高于HIV视网膜病变组和眼部正常组。 结论： 较之于AIDS眼部正常患者和HIV视网膜病变患者，AIDS患者的CMVR严重影响患者视力，3个组在细胞因子MIP-1b、IL-6、TNF-α、VEGF、IL-8、MCP-1、IP-10的含量不同可能与单核巨噬细胞系统（趋化因子、炎症因子、血管内皮因子）和Th1细胞反应活跃有关。（中华眼科杂志，2017，53：746-752）.

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