These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A simple method for the purification of dipeptidyl aminopeptidase IV from porcine kidney using HPLC.
    Author: Hutchinson DW, Woolfitt AR.
    Journal: Biomed Chromatogr; 1986 Aug; 1(4):147-50. PubMed ID: 2906548.
    Abstract:
    Dipeptidyl aminopeptidase IV (E.C. 3.4.14.5) has been purified to homogeneity using "soft-column" chromatography on Sephadex G-200, DEAE-cellulose, gly-pro-AH-Sepharose and finally HPLC on TSK G-3000SW. The purification by HPLC is fast and gives a better yield of pure enzyme than procedures which have been described previously. The enzyme obtained in this manner has a high specific activity (86 U/mg using a saturating level of gly-pro-4-nitroanilide as substrate) and was free of contaminating peptidase activities. Our preparation of DAP IV is suitable for peptide sequencing studies by fast atom bombardment mass spectrometry.
    [Abstract] [Full Text] [Related] [New Search]