These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Multimer of the antimicrobial peptide Mytichitin-A expressed in Chlamydomonas reinhardtii exerts a broader antibacterial spectrum and increased potency.
    Author: Dong B, Cheng RQ, Liu QY, Wang J, Fan ZC.
    Journal: J Biosci Bioeng; 2018 Feb; 125(2):175-179. PubMed ID: 29066128.
    Abstract:
    In this study, an antimicrobial peptide composed of three tandem repeats of Mytichitin-A (3 × Mytichitin-A) with a designed molecular weight of approximately 25 kDa was expressed in the green alga Chlamydomonas reinhardtii. The yield of 3 × Mytichitin-A reached 0.28% of the total soluble protein of the 3 × Mytichitin-A-expressing transgenic cells and the expression level was stable following continuous passaging of the cells for six months. Compared to its natural and yeast-produced recombinant counterparts, which showed a very low level of growth inhibition of gram-negative bacteria, the 3 × Mytichitin-A inhibited the growth of gram-negative bacteria at a minimum inhibition concentration value ranging between 60 and 80 μg/ml. The expressed 3 × Mytichitin-A did not show toxicity to HEK293 cells. Its bioactivity was hardly affected by temperature and pH but was impaired to some extent by the proteinase treatment. Taken together, our study showed that C. reinhardtii can be used as a cellular factory to produce bioactive Mytichitin-A in a multimeric format.
    [Abstract] [Full Text] [Related] [New Search]