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  • Title: Determination of S-Adenosylmethionine Decarboxylase Activity in Plants.
    Author: Tiburcio AF, Alcázar R.
    Journal: Methods Mol Biol; 2018; 1694():123-128. PubMed ID: 29080162.
    Abstract:
    The synthesis of spermidine, spermine and thermospermine requires the addition of aminopropyl groups from decarboxylated S-adenosyl-methionine (dSAM). The synthesis of dSAM is catalyzed by S-adenosylmethionine decarboxylase. dSAM levels are usually low, which constitutes a rate-limiting factor in the synthesis of polyamines. In this chapter, we provide a protocol for the determination of SAMDC activity in plants through the detection of radiolabelled CO2 released during the SAMDC reaction.
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