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  • Title: Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae.
    Author: Lin J, Huang N, Wang H, Fu Q, Wang E, Li P, Yang L, Luo X, Liu X, Liu Z.
    Journal: Immunobiology; 2018 Feb; 223(2):246-251. PubMed ID: 29102047.
    Abstract:
    House dust mite (HDM) allergen is a major cause of allergic disease. In this study, two-dimensional immunoblot and Matrix-Assisted Laser Desorption Ionization tandem Time-of-flight mass spectrometry (MALDI-TOF-MS) were used to identify Der f 31. After Der f 31 was cloned, expressed and purified, skin prick test (SPT), Immune inhibitory assays, Western blot, ELISA and asthmatic mouse model were employed to examine the allergenicity of recombinant Der f 31. The gene of Der f 31 includes 447 bps, and encoded 148 amino acids. Positive responses of SPT to r-Der f 31 were 32.5% in 43 HDM-allergic patients. r-Der f 31 can induce allergic pulmonary inflammation in the mouse model. In conclusion, Der f 31 is a novel subtype of dust mite allergens.
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