These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Development & validation of reversed phase HPLC method for quantification of water insoluble API.
    Author: Yasmin R, Shoaib MH, Muhammad IN, Bashir L, Naz S, Ali H, Zafar F, Siddiqui F.
    Journal: Pak J Pharm Sci; 2017 Sep; 30(5(Supplementary)):2021-2024. PubMed ID: 29105638.
    Abstract:
    In the present work a specific, accurate, precise, and reproducible UV-HPLC method was developed and validated for the analysis of Aceclofenac. This method involved elution of Aceclofenac in a mobile phase which is composed of buffer pH 6.8 (i.e. using 0.01N KH2PO4) and HPLC grade Acetonitrile (60:40). Separation of the analyte was achieved using HPLC isocratic pump attached to the UV-VIS detectorC18, guard column and C18 column. The injection volume was 20μL, detected at 274 nm; flow rate: 1mL/min. Standard calibration curve was measured and found linear from 0.1 to 40μg/ml. The validation parameters were measured according to FDA guidelines and successful results were obtained. The presented analytical method could be employed for pharmacokinetic studies.
    [Abstract] [Full Text] [Related] [New Search]