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  • Title: Thermodynamic Activity-Based Progress Curve Analysis in Enzyme Kinetics.
    Author: Pleiss J.
    Journal: Trends Biotechnol; 2018 Mar; 36(3):234-238. PubMed ID: 29107319.
    Abstract:
    Macrokinetic Michaelis-Menten models based on thermodynamic activity provide insights into enzyme kinetics because they separate substrate-enzyme from substrate-solvent interactions. Kinetic parameters are estimated from experimental progress curves of enzyme-catalyzed reactions. Three pitfalls are discussed: deviations between thermodynamic and concentration-based models, product effects on the substrate activity coefficient, and product inhibition.
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