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  • Title: A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330.
    Author: Vuillemin M, Grimaud F, Claverie M, Rolland-Sabaté A, Garnier C, Lucas P, Monsan P, Dols-Lafargue M, Remaud-Siméon M, Moulis C.
    Journal: Carbohydr Polym; 2018 Jan 01; 179():10-18. PubMed ID: 29111031.
    Abstract:
    A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691s-1 and a half-life time of 111h at 30°C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1×109g·mol-1 that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.
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