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  • Title: Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe.
    Author: Sato SI, Yatsuzuka K, Katsuda Y, Uesugi M.
    Journal: Methods Mol Biol; 2018; 1649():305-318. PubMed ID: 29130206.
    Abstract:
    Live-cell imaging of mRNA dynamics is increasingly important to understanding spatially restricted gene expression. We recently developed a convenient and versatile method that uses a gene-specific RNA aptamer and a fluorescent probe to enable spatiotemporal imaging of endogenous mRNAs in living cells. The method was validated by live-cell imaging of the endogenous mRNA of β-actin. The new RNA-imaging technology might be useful for live-cell imaging of any RNA molecules.
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