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  • Title: [Determination of serum and plasma erythropoietin in mice with phenylhydrazine-induced anemia].
    Author: Manakova TE, Setkov NA.
    Journal: Biull Eksp Biol Med; 1989 Jan; 107(1):21-4. PubMed ID: 2914166.
    Abstract:
    The simple, specific and sensitive erythropoietin bioassay in serum and plasma from phenylhydrazine treated mice is described, based on H3-thymidine incorporation into divided hemopoietic cells. Spleen cells taken from mice on the third day following the second of 2 daily injections of phenylhydrazine were cultured in 24 hours in the presence of test material. Following incubation for 2 hours with H3-thymidine solution radioactivity was measured.
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