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  • Title: HMG proteins released from the chromatin following incubation of mammalian nuclei with ethidium bromide.
    Author: Pani B, Plossi P, Russo E.
    Journal: Exp Cell Res; 1989 Feb; 180(2):557-62. PubMed ID: 2914585.
    Abstract:
    The low-molecular-mass high-mobility group (HMG) chromosomal proteins, namely HMG-14, HMG-17, and HMG-I, which have been found in several proliferating tissues, are released following incubation of nuclei isolated from young rat thymus and from human placenta in a low ionic strength medium containing the intercalating agent ethidium bromide. The amount of HMG proteins released is drug concentration-dependent, but at very high concentrations (20-40 mM) other low- and high-molecular-mass proteins, and even histones, are released. These results suggest a very weak interaction of the HMG proteins with DNA, so that they can be easily detached from the chromatin as a consequence of the interaction of DNA with the intercalating agent.
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