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  • Title: Recovery of motile sperm using the migration-sedimentation technique in an in-vitro fertilization-embryo transfer programme.
    Author: Lucena E, Lucena C, Gómez M, Ortiz JA, Ruiz J, Arango A, Diaz C, Beuerman C.
    Journal: Hum Reprod; 1989 Feb; 4(2):163-5. PubMed ID: 2918068.
    Abstract:
    Sperm washing techniques, based on the swim-up principle used before inseminating the human oocyte in in-vitro fertilization and embryo transfer programmes (IVF-ET), usually require prior centrifugation which causes damage to the sperm cell. A technique is described for separating sperm at laboratory temperature based on sperm migration--sedimentation principles, using two concentric tubes and recovering 70-90% forward-moving cells. A group of 17 patients is presented who were managed with this method. The results were 85% fertilization rate, 4% polyspermia and six clinical pregnancies.
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