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  • Title: Maturation of chloride-bicarbonate exchange in rat ileal brush border membrane vesicles.
    Author: Riedel BD, Ghishan FK.
    Journal: Pediatr Res; 1989 Feb; 25(2):189-93. PubMed ID: 2919135.
    Abstract:
    The present studies were designed to examine the ontogeny of the Cl- -HCO3- exchange process in the ileum of suckling (2 wk old), weanling (3 wk old), and adult (6 wk old) rats using well-validated brush border membrane vesicle techniques. The purity of the vesicle preparation was verified by demonstrating that the activity of brush border marker enzyme activity was enriched 30-fold compared to the cell homogenate. Glucose uptake experiments demonstrated an overshoot phenomenon under Na+ gradient conditions, but not in the absence of Na+. Chloride uptake varied inversely with the extravesicular osmolarity, demonstrating that transport was into an osmotically sensitive space. Membrane binding accounted for only 20% of total uptake. An inwardly-directed pH gradient (pHout/pHin = 5.2/7.5) produced overshoot Cl- uptake in all age groups. The magnitude of overshoot was greatest in suckling rats and declined with advancing age. Addition of an outwardly-directed HCO3- gradient yielded further stimulation of Cl- uptake in suckling and weanling animals but could not be demonstrated in the adult. The majority of Cl- uptake proceeded via an electroneutral exchange process. However, a conductive component was present, as demonstrated by enhancement of uptake when the vesicles were rendered positive inside by a K+ gradient and valinomycin, as compared with voltage clamp conditions. At 5 mM concentration, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, an inhibitor of anion exchange, inhibited Cl- uptake by 62, 44, and 33%, respectively in suckling, weanling, and adult rats. The initial rate of uptake was linear for 8 s.(ABSTRACT TRUNCATED AT 250 WORDS)
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