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  • Title: Harvesting and Characterization of Syncytial Nuclear Aggregates Following Culture of First Trimester Human Placental Explants.
    Author: Pantham P, Chamley LW.
    Journal: Methods Mol Biol; 2018; 1710():155-163. PubMed ID: 29197001.
    Abstract:
    There is currently no effective method to study multinucleated trophoblast debris extruded from the syncytiotrophoblast into the maternal circulation. In Chapter 9 , an in vitro placental explant culture model to generate trophoblast debris was described. Here, we detail the method utilized to isolate individual large multinucleated syncytial nuclear aggregates (SNAs) that are extruded from the syncytiotrophoblast following the culture of first trimester human placental explants. Syncytial nuclear aggregates have been observed in the peripheral maternal circulation as early as 6 weeks' gestation and may play a role in tolerating the maternal immune system during pregnancy. Conversely, aberrant cell death processes in the syncytiotrophoblast due to various maternal factors leading to the extrusion of SNAs that are altered in nature have been implicated in the development of preeclampsia. The methods described herein allow for the isolation and harvest of SNAs without other types of extruded trophoblast debris and can be used to investigate the effect of various maternal factors on the nature of SNAs extruded from the placenta in vitro.
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