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  • Title: Expression of Two RpoH Sigma Factors in Sinorhizobium meliloti upon Heat Shock.
    Author: Mitsui H, Minamisawa K.
    Journal: Microbes Environ; 2017 Dec 27; 32(4):394-397. PubMed ID: 29199214.
    Abstract:
    The plant symbiotic α-proteobacterium Sinorhizobium meliloti has two RpoH-type sigma factors, RpoH1 and RpoH2. The former induces the synthesis of heat shock proteins and optimizes interactions with the host. Using a Western blot analysis, we examined time course changes in the intracellular contents of these factors upon a temperature upshift. The RpoH1 level was relatively high and constant, suggesting that its regulatory role in the heat shock response is attained through the activation of the pre-existing RpoH1 protein. In contrast, the RpoH2 level was initially undetectable, and gradually increased. These differential patterns reflect the functional diversification of these factors.
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