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  • Title: A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin.
    Author: Neselioglu S, Ergin M, Erel O.
    Journal: Anal Sci; 2017; 33(12):1339-1344. PubMed ID: 29225221.
    Abstract:
    A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.
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