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  • Title: [Micromethods for the Widal reaction].
    Author: Mamat H, Kroh G.
    Journal: Z Gesamte Hyg; 1989 Jan; 35(1):39-40. PubMed ID: 2922944.
    Abstract:
    Micro methods are described at the example of Salmonella and Yersinia-O-Widal and the Salmonella-H-Widal. The reaction is performed by micro titre plates with painted antigens. Basic Fuchsin had been used. Other antigen concentrations are partially required as to the tubule agglutination. The parallel comparison of the tubule and micro methods with test serum resulted in an important variation range of the tubule method. By subjective registration the rates may vary between 3 to 6 titre stages, same results in the micro method amount to 1 titre stage only. Thus by means of the micro method, the evidence of the Widal reaction is improved reducing at the same time expenses for material and work.
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